Transcriptional expression levels and biochemical markers of oxidative stress in Mytilus galloprovincialis exposed to nickel and heat stress.
Identifieur interne : 000059 ( Main/Exploration ); précédent : 000058; suivant : 000060Transcriptional expression levels and biochemical markers of oxidative stress in Mytilus galloprovincialis exposed to nickel and heat stress.
Auteurs : Mohamed Banni [Tunisie] ; Attig Hajer [Tunisie] ; Suzanna Sforzini [Italie] ; Caterina Oliveri [Italie] ; Hamadi Boussetta [Tunisie] ; Aldo Viarengo [Italie]Source :
- Comparative biochemistry and physiology. Toxicology & pharmacology : CBP [ 1532-0456 ] ; 2014.
English descriptors
- KwdEn :
- Animals, Biomarkers (metabolism), Female, Gene Expression Regulation, Heat-Shock Response (drug effects), Heat-Shock Response (physiology), Hot Temperature (adverse effects), Mytilus, Nickel (toxicity), Oxidative Stress (drug effects), Oxidative Stress (physiology), Transcription, Genetic (drug effects), Transcription, Genetic (physiology).
- MESH :
- chemical , metabolism : Biomarkers.
- adverse effects : Hot Temperature.
- drug effects : Heat-Shock Response, Oxidative Stress, Transcription, Genetic.
- physiology : Heat-Shock Response, Oxidative Stress, Transcription, Genetic.
- chemical , toxicity : Nickel.
- Animals, Female, Gene Expression Regulation, Mytilus.
Abstract
The present study aims to evaluate transcriptional expression levels and biochemical markers of oxidative stress responses to nickel (Ni) exposure along with heat stress gradient in a mussel (Mytilus galloprovincialis). For this purpose, we investigated the response of oxidative stress markers, metallothionein accumulation and gene expression in digestive gland of mussels exposed to a sublethal concentration of Ni (2.5μM) along with a temperature gradient (18°C, 22°C, and 26°C) for 24h and 72h. Ni digestive gland uptake was evaluated after the exposure periods. Co-exposure to Ni and higher temperature (26°C) for 72h significantly decreased the antioxidant enzyme activities termed as catalase (CAT), superoxide dismutase (SOD) and glutathione-S-transferase (GST) and caused a pronounced increase of lipofuscin and neutral lipid (NL) accumulation. Ni-uptake was different with respect to the exposure periods and temperatures in Ni-exposed mussels. Sod, cat, gst, mt-10 and mt20 gene expression levels showed a substantial increased pattern in animals exposed for one day to heat stress compared to the control condition (18°C). The same pattern but with highest level was registered in animals co-exposed to Ni and temperatures within one day. Three days exposure to 18°C, 22°C and 26°C, resulted in a significant decrease in mRNA abundance of cat, gst and sod and a significant down-regulation of mts targets (22°C and 26°C). Our data provide new insights into the importance of the early protective response of oxidative stress related-gene expression and regulation in mussels challenging heat stress and sublethal Ni concentration.
DOI: 10.1016/j.cbpc.2013.11.005
PubMed: 24291086
Affiliations:
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For this purpose, we investigated the response of oxidative stress markers, metallothionein accumulation and gene expression in digestive gland of mussels exposed to a sublethal concentration of Ni (2.5μM) along with a temperature gradient (18°C, 22°C, and 26°C) for 24h and 72h. Ni digestive gland uptake was evaluated after the exposure periods. Co-exposure to Ni and higher temperature (26°C) for 72h significantly decreased the antioxidant enzyme activities termed as catalase (CAT), superoxide dismutase (SOD) and glutathione-S-transferase (GST) and caused a pronounced increase of lipofuscin and neutral lipid (NL) accumulation. Ni-uptake was different with respect to the exposure periods and temperatures in Ni-exposed mussels. Sod, cat, gst, mt-10 and mt20 gene expression levels showed a substantial increased pattern in animals exposed for one day to heat stress compared to the control condition (18°C). The same pattern but with highest level was registered in animals co-exposed to Ni and temperatures within one day. Three days exposure to 18°C, 22°C and 26°C, resulted in a significant decrease in mRNA abundance of cat, gst and sod and a significant down-regulation of mts targets (22°C and 26°C). Our data provide new insights into the importance of the early protective response of oxidative stress related-gene expression and regulation in mussels challenging heat stress and sublethal Ni concentration.</div></front></TEI><affiliations><list><country><li>Italie</li><li>Tunisie</li></country></list><tree><country name="Tunisie"><noRegion><name sortKey="Banni, Mohamed" sort="Banni, Mohamed" uniqKey="Banni M" first="Mohamed" last="Banni">Mohamed Banni</name></noRegion><name sortKey="Boussetta, Hamadi" sort="Boussetta, Hamadi" uniqKey="Boussetta H" first="Hamadi" last="Boussetta">Hamadi Boussetta</name><name sortKey="Hajer, Attig" sort="Hajer, Attig" uniqKey="Hajer A" first="Attig" last="Hajer">Attig Hajer</name></country><country name="Italie"><noRegion><name sortKey="Sforzini, Suzanna" sort="Sforzini, Suzanna" uniqKey="Sforzini S" first="Suzanna" last="Sforzini">Suzanna Sforzini</name></noRegion><name sortKey="Oliveri, Caterina" sort="Oliveri, Caterina" uniqKey="Oliveri C" first="Caterina" last="Oliveri">Caterina Oliveri</name><name sortKey="Viarengo, Aldo" sort="Viarengo, Aldo" uniqKey="Viarengo A" first="Aldo" last="Viarengo">Aldo Viarengo</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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